GENETIC CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF BACILLUS ANTHRACIS STERNE STRAIN BY 16S RRNA GENE SEQUENCING
نویسندگان
چکیده
Anthrax, caused by Bacillus anthracis, is a fatal disease and all the warm bloodedanimals are susceptible to this disease. Many livestock populated areas in Pakistan endemic forAnthrax (Thappa Karthikeyan 2001), therefore population annually vaccinatedagainst Anthrax. Veterinary Research Institute (VRI) has been producing Anthrax Spore Vaccine(ASV) for couple of decades. The current study was designed molecular characterization themicroorganism used vaccine production (Bacillus anthracis Sterne strain) its phylogeneticrelationship with related strains species. For purpose, 16S rRNA gene amplifiedand sequenced found 100% genetically similar reference sequence respect 16SrRNA gene. Phylogenetic analysis revealed that strain showed close phylogenecity withBacillus 1144 V77-NP-1R. Moreover, organism wasfound closer cereus than mycoides thuringiensis withrespect sequence.
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ژورنال
عنوان ژورنال: Pakistan journal of science
سال: 2023
ISSN: ['0030-9877', '2411-0930']
DOI: https://doi.org/10.57041/pjs.v68i1.124